Technotes, Publications, Technology platforms and FAQs
Publications and Technotes
ACS Biomarker aims to advance translational research by publishing high-quality data in peer reviewed journals. See our technotes for details on our measurement and analysis pipeline.
Prognostic value of circulating microRNAs on heart failure-related morbidity and mortality in two large diverse cohorts of general heart failure patients.
Bayés-Genis A. et al. Eur J Heart Fail. 2017 Sep 26
Serially measured circulating microRNAs and adverse clinical outcomes in patients with acute heart failure.
van Boven N. et al. Eur J Heart Fail. 2017 Sep 26
Circulating miRNA Analysis Guidelines
De Ronde et al. RNA. 2017 May;23(5):811-821
miR-22-3p in heart failure
Van Boven et al. Int J Cardiol. 2017 May 15;235:124-132
Creemers EE et al. Circ Res. 2012 Feb 3;110(3):483-95
Tijsen et al. Circ Res. 2010 Apr 2;106(6):1035-9.
Cardio-Onco Biomarker Panel
RNA profiling platform
To obtain high quality results we have chosen the superior miRNA platform of Quantabio for our biomarker services. This platform offers a cost-effective method to measure multiple RNA molecules from a single RT reaction. The open technology provides flexibility to design your own assay primers and eliminates inefficient stem-loop priming and costly hydrolysis probes. It has been reported as one of the best miRNA profiling platforms and guarantees to deliver reliable results.
Is every sample suitable for RNA profiling?RNA is present in most tissues and we have protocols for extraction and detection of RNA from a wide variety of samples. However, we cannot profile RNA in blood collected in heparin tubes as heparin inhibits the PCR measurement. Blood from patients that received heparin infusion should be tested in a pilot experiment.
How stable are RNA molecules?In contrast to messenger RNAs, non-coding RNAs such as miRNAs, lncRNAs and circRNAs are extremely stable. In the circulation they are present in extracellular vesicles (e.g. exosomes) wich protects them from degradation.
How do I submit my samples?In our technote section you find a sample submission technote and a sample submission form.
What method is used for RNA profiling?At ACS Biomarker we use quantitative PCR (qPCR) as method for RNA detection.